APPLICATION OF MICROSATELLITE-BASED PARENTAGE VERIFICATION USING VeriSireTM IN SELECTED CATTLE FARMS IN THE PHILIPPINES

Abstract

Parentage verification using microsatellite markers is yet to be fully appreciated in the local cattle industry. Breeder experiences have proven that parentage testing, in combination with well-run breeding programs, can ensure accurate pedigrees which are necessary for genetic improvement and to avoid unintentional inbreeding. However, keeping such records is a widespread challenge among cattle raisers specifically in keeping their bull records. Thus, this study aims to validate the pedigree of animals in farms with different breeding methods using VeriSire^TM - a DNA-based test to verify the true sire of a calf. Blood and hair follicle samples were collected from four local cattle farms. Subsequent processes of DNA extraction, PCR amplification using 16 markers pooled into 4 panels, gel electrophoresis, capillary electrophoresis, and parentage assignment were performed. The study underscores the practicality of using gDNA extracted from hair follicle samples as an alternative to blood samples. Our results revealed that through parentage verification, the pedigree information of the target animals can be corrected using VeriSire^TM. The analysis identified the true sire of 20 animals in a farm utilizing more than 20 bulls for breeding. In three other farms that utilize AI and a combination of both, sire mismatches were identified, and the pedigree of 26 animals was corrected and validated. These results are strong indications that either one breeding method can be compromised when breeding programs are not properly implemented. This research emphasizes the importance and efficacy of rigorous pedigree verification methods in ensuring the integrity and genetic progress of breeding programs in the livestock industry.