DIACYLGLYCEROL ACYLTRANSFERASE 1 (DGAT1), STEAROYL-COA DESATURASE 1 (SCD1), AND FATTY ACID SYNTHASE (FASN) GENOTYPES AND THEIR ASSOCIATION WITH FAT PERCENTAGE AND MAJOR FATTY ACIDS IN COLOSTRUM AND MILK FROM HOLSTEIN × AUSTRALIAN FRIESIAN SAHIWAL COWS

Abstract

This study analyzed the association of bovine lipogenic gene markers – diacylglycerol acyltransferase 1 (DGAT1), stearoyl-CoA desaturase 1 (SCD1), and fatty acid synthase (FASN), with fat percentage and major fatty acids (myristic acid C14:0, palmitic acid C16:0, oleic acid C18:1n-9, and linoleic acid C18:2n-6) in 180 colostrum and milk samples collected from 45 Holstein × Australian Friesian Sahiwal (AFS) cows in a local dairy farm in Bay, Laguna. Genotypes for DGAT1 (AK, KK), SCD1 (AA, AV, VV), and FASN (CT, TT) were determined through polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) using DNA extracted from hair follicles. The AK genotype of DGAT1 gene was associated (P<0.05) with higher fat content in colostrum and higher C14:0 in milk than for the KK genotype. The AV genotype of SCD1 gene was associated (P<0.05) with the highest fat content and C18:1n-9 in colostrum and highest C16:0 and C18:0 in milk, compared to AA or VV genotypes. The CT genotype of the FASN gene was associated (P<0.05) with higher C14:0 in colostrum compared to CC genotype. This study showed polymorphisms in DGAT1, SCD1, and FASN genotypes and their significant associations with fat content and some major fatty acids in colostrum and milk.