RELATIONSHIP OF FIBRONECTIN 1 (FN1) TO THE EXTENDED SEMEN SHELF LIFE OF DUROC AND PHILIPPINE NATIVE BOAR-QUEZON AT DIFFERENT STORAGE TEMPERATURES

Ermie B. Mariano, Jr., Geleo A. Dichoso, Consuelo Amor S. Estrella, Percival P. Sangel

Abstract


An investigation to assess the seminal plasma protein level of Fibronectin 1 (FN1) as indicator of semen self-life was done using fresh semen from Quezon and Duroc boars diluted with commercially available medium-term extender and stored at either room (22-25℃) or refrigerated (4-8℃) temperature. The seminal plasma FN1 protein levels were quantified using western blot followed by a chemiluminescent screening. Meanwhile, sperm motility parameters and morphology were assessed using a computer-assisted sperm analyzer (CASA) every 4 hours until acceptable values for good extended semen quality were observed. The contrast between the shelf-lives of extended semen between breeds was not significant at room or refrigerated temperature. Moreover, the comparison of extended semen shelf life between the two storage temperatures yielded a non-significant difference for both Quezon and Duroc boars. Association between FN1 levels and extended semen shelf life revealed a trend (P< 0.10), where a strong relationship in room storage was observed. This study demonstrated comparable characteristics of extended semen from Quezon and Duroc boars, and the potential use of FN1 as a marker for boar semen shelf life analysis.

Keywords


Duroc, Fibronectin, Philippine native boar, semen, shelf life

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