CONJUGATED LINOLEIC ACID PRODUCTION OF Lactobacillus plantarum BIOTECH 1066 IN MILK

Abstract

This study was conducted to quantify conjugated linoleic acid production (CLA) of Lactobacillus plantarum BIOTECH 1066 as well as its viable count and per­cent lactic acid in sterile cow, buffalo and goat milk. The sterile medium was inoculated with 1% L. plantarum BIOTECH 1066 and then incubated at 370C for 18 hours. Lipids were extracted from the reaction mixture with choloro­form/methanol (1:2 v/v) and were analyzed using a GC-14 gas chromatograph. Triplicate data were statistically analyzed using t-test and analysis of variance (ANOVA) in completely randomized design at 5% level of significance. Com­parison of treatment means was done using Least Significant Difference. Viable cell count (cfu/ml) and developed acidity of L. plantarum 1066 had no significant differences after 18 hours of incubation on different media while CLA produced has increased significantly. Using different milk media, L. plantarum 1066 was able to produce significant amounts of CLA (0.32 to 0.91 mg/ml) with signifi­cant concentration of 0.62 mg/ml synthesized in buffalo’s milk. This could be attributed to the relatively high fat content of buffalo milk (7.2%).